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Quantitative elemental imaging of octopus stylets using PIXE and the nuclear microprobe


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Doubleday, ZA, Belton, D, Pecl, GT and Semmens, JM 2007 , 'Quantitative elemental imaging of octopus stylets using PIXE and the nuclear microprobe' , Nuclear Instruments and Methods in Physics Research B, vol. 266, no. 1 , pp. 67-72 , doi: 10.1016/j.nimb.2007.10.027.

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By utilising targeted microprobe technology, the analysis of elements incorporated within the hard bio-mineralised structures of
marine organisms has provided unique insights into the population biology of many species. As hard structures grow, elements from
surrounding waters are incorporated effectively providing a natural ‘tag’ that is often unique to the animal’s particular location or
habitat. The spatial distribution of elements within octopus stylets was investigated, using the nuclear microprobe, to assess their potential
for determining dispersal and population structure in octopus populations. Proton Induced X-ray Emission (PIXE) was conducted
using the Dynamic Analysis method and GeoPIXE software package, which produced high resolution, quantitative elemental maps of
whole stylet cross-sections. Ten elements were detected within the stylets which were heterogeneously distributed throughout the microstructure.
Although Ca decreased towards the section edge, this trend was consistent between individuals and remained homogeneous in
the inner region of the stylet, and thus appears a suitable internal standard for future microprobe analyses. Additional analyses used to
investigate the general composition of the stylet structure suggested that they are amorphous and largely organic, however, there was
some evidence of phosphatic mineralisation. In conclusion, this study indicates that stylets are suitable for targeted elemental analysis,
although this is currently limited to the inner hatch region of the microstructure.

Item Type: Article
Authors/Creators:Doubleday, ZA and Belton, D and Pecl, GT and Semmens, JM
Journal or Publication Title: Nuclear Instruments and Methods in Physics Research B
ISSN: 0168-583X
DOI / ID Number: 10.1016/j.nimb.2007.10.027
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