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A molecular approach to identify prey of the southern rock lobster
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Redd, KS, Jarman, SN, Frusher, SD and Johnson, CR 2008
, 'A molecular approach to identify prey of the southern rock lobster'
, Bulletin of Entomological Research, vol. 98, no. 3
, pp. 233-238
, doi: 10.1017/S0007485308005981.
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2008_Redd,_Jarm...pdf | Request a copy Full text restricted Available under University of Tasmania Standard License. |
Official URL: http://dx.doi.org/10.1017/S0007485308005981
Abstract
We demonstrate the use of molecular techniques to detect specific prey
consumed by the southern rock lobster ( Jasus edwardsii). A quick and non-lethal
method was used to collect rock lobster faecal material and a molecular protocol
was employed to isolate prey DNA from faecal samples. The isolated DNA was
amplified using the polymerase chain reaction (PCR) with PCR primers designed
to target specific prey items. Feeding experiments determined that DNA from
black-lipped abalone (Haliotis rubra) and sea urchins (Centrostephanus rodgersii and
Heliocidaris erythrogramma) can be detected in rock lobster faecal samples within
seven hours and remains present for up to 60 h after ingestion.
Item Type: | Article |
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Authors/Creators: | Redd, KS and Jarman, SN and Frusher, SD and Johnson, CR |
Keywords: | Jasus edwardsii, Haliotis rubra, polymerase chain reaction (PCR), prey detection, rock lobster diet |
Journal or Publication Title: | Bulletin of Entomological Research |
ISSN: | 0007-4853 |
DOI / ID Number: | 10.1017/S0007485308005981 |
Item Statistics: | View statistics for this item |
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