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Determination of ribavirin in human serum and plasma by capillary electrophoresis

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Breadmore, MC, Theurillat, R and Thormann, W 2004 , 'Determination of ribavirin in human serum and plasma by capillary electrophoresis' , Electrophoresis, vol. 25, no. 10-11 , pp. 1615-1622 , doi: 10.1002/elps.200305819.

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Abstract

The electrophoretic separation of ribavirin and 5-methylcytidine (internal standard) by capillary electrophoresis was examined. Separation was achieved using reverse polarity in a 100 mM borate electrolyte, pH 9.1, with 5 mM spermine added to reduce the electroosmotic flow. Sample preparation based on acetonitrile protein precipitation was found to be unsuitable for ribavirin analysis in patient samples due to insufficient sensitivity and interferences. Solid-phase extraction employing phenyl boronic acid cartridges provided cleaner separations. Using this approach with 500 {micro}L sample and reconstitution of the dried extract into 100 {micro}L of 33% v/v 100 mM phosphate buffer, pH 6.4 / 67% v/v acetonitrile, the detection and quantitation limits were determined to be 0.05 and 0.10 {micro}g/mL, respectively, a sensitivity that is suitable for therapeutic drug monitoring of ribavirin in human plasma and serum samples. The method was validated and compared to a high-performance liquid chromatography (HPLC) method, showing excellent agreement between the two for a set of samples that stemmed from patients being treated with ribavirin and interferon-{alpha}-2b for a hepatitis C virus infection.

Item Type: Article
Authors/Creators:Breadmore, MC and Theurillat, R and Thormann, W
Keywords: Capillary electrophoresis • High-performance liquid chromatography • Ribavirin • Therapeutic drug monitoring
Journal or Publication Title: Electrophoresis
ISSN: 0173-0835
DOI / ID Number: 10.1002/elps.200305819
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Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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