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Abstract

Abstract The stimulatory effect of vasomodulatory natriuretic peptide hormones on macrophages
and peripheral blood leucocytes in mammals is well-established. However, the relationship
in lower vertebrates has not been characterised. Expression of atrial natriuretic peptide,
ventricular natriuretic peptide and C-type natriuretic peptide-1, and the guanylyl cyclaselinked
(GC) natriuretic peptide receptor-A and -B-type receptors (NPR-A and NPR-B, respectively)
was determined by PCR from the mRNA of rainbow trout head kidney leucocytes yielding
gene fragments with 100% homology to the same respective natriuretic peptide and NPR-A and -B
sequences obtained from other rainbow trout tissues. A mixed population of isolated rainbow
trout head kidney leucocytes was stimulated in vitro with trout atrial natriuretic peptide (specific
NPR-A agonist) and trout C-type natriuretic peptide (NPR-A and -B agonist) as well as the
cGMP agonist 8-bromo-cGMP or the GC inhibitor 8-bromo-phenyl-eutheno-cGMP. Respiratory
burst was stimulated by trout atrial natriuretic peptide, trout C-type natriuretic peptide-1
and 8-bromo-cGMP in a dose dependant manner with the highest activity as a result of stimulation
with trout C-type natriuretic peptide-1 in excess of that achieved by phorbol myristate acetate
(PMA). Equimolar concentrations of the inhibitor, inhibited the respiratory burst caused
by the natriuretic peptides and 8-bromo-cGMP. The natriuretic peptide receptors on rainbow

Item Type:	Article
Authors/Creators:	Powell, MD and McWilliam, H and McLeod, J and Nankervis, S and Butler, R and Toop, T
Keywords:	Natriuretic peptides; Leukocytes; Rainbow trout; Respiratory burst
Journal or Publication Title:	Fish and Shellfish Immunology
ISSN:	1050-4648
DOI / ID Number:	https://doi.org/10.1016/j.fsi.2007.08.003
Additional Information:	The definitive version is available at http://www.sciencedirect.com
Item Statistics:	View statistics for this item

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