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Isolation, amplification, and identification of ancient copepod DNA from lake sediments


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Bissett, A, Gibson, JAE, Jarman, SN, Swadling, KM and Cromer, L 2005 , 'Isolation, amplification, and identification of ancient copepod DNA from lake sediments' , Limnology and Oceanography: Methods, vol. 3 , pp. 533-542 .

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Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic
features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a
method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed
in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA
sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity.
Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP.
Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified
in the sediments matched those of extant lake populations, but analysis of early-mid Holocene sediments
from one lake revealed a species that is not present today. We were able to recover copepod DNA from core samples
stored at -20 degrees C, at 4 degrees C, and preserved with polyethyleneglycol.

Item Type: Article
Authors/Creators:Bissett, A and Gibson, JAE and Jarman, SN and Swadling, KM and Cromer, L
Keywords: ancient DNA, copepod, Antarctica, lake, Boeckella poppei, Paralabidocera antarctica
Journal or Publication Title: Limnology and Oceanography: Methods
ISSN: 1541-5856
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