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A microsatellite study on outcrossing rates and contamination in an Eucalyptus globulus breeding arboretum


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Rao, H, Patterson, B, Potts, BM and Vaillancourt, RE 2008 , 'A microsatellite study on outcrossing rates and contamination in an Eucalyptus globulus breeding arboretum' , Journal of Forestry Research, vol. 19, no. 2 , pp. 136-140 , doi: 10.1007/s11676-008-0023-6.

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Four pairs of microsatellite molecular polymorphism primers were used to analyse microsatellite fingerprints of 188 seedlings derived
from an open-pollinated progeny grafted Eucalyptus globulus breeding arboretum in Victoria, south-eastern Australia. The microsatellite
loci chosen for this study were highly polymorphic with the mean number of alleles per locus of 14.25. Individual mothers varied in their
outcrosssing rate estimate from 15% to 95%, the overall outcrossing level in the arboretum was 47.9% and the contamination rate was 17.6%.
The high selfing level was likely to result in marked inbreeding depression in the performance of open-pollinated seed lots. Open-pollinated
seeds collected from such arboreta are not advisable because of its low genetic quality, although such arboreta may be useful for the seed
production through large-scale manual pollination or collecting seeds only from trees or genotypes within the arboretum that have high outcrossing

Item Type: Article
Authors/Creators:Rao, H and Patterson, B and Potts, BM and Vaillancourt, RE
Keywords: E. globulus; breeding arboretum; microsatellite; outcrossing rate; pollen contamination
Journal or Publication Title: Journal of Forestry Research
ISSN: 1007-662X
DOI / ID Number: 10.1007/s11676-008-0023-6
Additional Information:

BM Potts. The original publication is available at

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