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Separation of antidepressants by capillary electrophoresis with in-line solid-phase extraction using a novel monolithic adsorbent


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Schaller, D, Hilder, EF and Haddad, PR 2006 , 'Separation of antidepressants by capillary electrophoresis with in-line solid-phase extraction using a novel monolithic adsorbent' , Analytica Chimica Acta, vol. 556, No. 1 , pp. 104-111 , doi: 10.1016/j.aca.2005.07.035.

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The separation of three selective serotonin reuptake inhibitors (SSRIs) by capillary electrophoresis (CE) with fully integrated solid-phase extraction (SPE) is described. Polymeric monolithic SPE modules were prepared in situ within a fused silica capillary from either butyl methacrylate-co-ethylene dimethacrylate or 3-sulfopropyl methacrylate-co-butyl methacrylate-co-ethylene dimethacrylate. Using a 1 cm SPE module placed at the inlet of the capillary, a mixture of sertraline, fluoxetine and fluvoxamine was extracted from aqueous solution by applying a simple pressure rinse. Under pressure-driven conditions, efficient elution was possible from both SPE materials investigated using 50 mM phosphate buffer, pH 3.5 in acetonitrile (20/80, v/v). Two different strategies were investigated for the efficient elution and subsequent CE separation. Injection of an aqueous sample plug directly into the non-aqueous elution/separation buffer was found to be unsuitable with poor elution profiles observed in the electrodriven mode. Alternatively, a sample plug equivalent to several capillary volumes could be injected by pressure followed by filling the capillary with the non-aqueous elution/separation buffer from the outlet end using a combination of pressure and electrodriven flow. Using a neutral monolith, efficient elution/separation was not possible due to an unstable electroosmotic flow (EOF), however, by adding the ionisable monomer, 3-sulfopropyl methacrylate to the SPE module to increase and stabilise the EOF, it was possible to achieve efficient elution from the SPE module, followed by baseline separation by CE using a 200 mM acetate buffer, pH 3.5 in acetonitrile (10/90, v/v). With enrichment factors of over 500 achieved for each of the analytes this demonstrates the potential of in-line SPE-CE for the sensitive analysis of these drugs.

Item Type: Article
Authors/Creators:Schaller, D and Hilder, EF and Haddad, PR
Keywords: Polymer monolith; In-line solid-phase extraction; Capillary electrophoresis; Drug analysis
Journal or Publication Title: Analytica Chimica Acta
ISSN: 0003-2670
DOI / ID Number: 10.1016/j.aca.2005.07.035
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