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Early ovule development following self- and cross pollination in Eucalyptus globulus Labill. ssp. globulus

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Pound, LM, Wallwork, MAB, Potts, BM and Sedgley, M 2002 , 'Early ovule development following self- and cross pollination in Eucalyptus globulus Labill. ssp. globulus' , Annals of Botany, vol. 89 , pp. 613-620 , doi: 10.1093/aob/mcf089.

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Abstract

The study was conducted to identify the self-incompatibility mechanism in Eucalyptus globulus ssp. globulus. Controlled self- and cross-pollinations were conducted on individual flowers from three mature trees that had self-incompatibility levels of 76, 99·6 and 100 %. Flowers were harvested at 4, 6 and 8 weeks after pollination. Embryology was investigated by bright field microscopy on material harvested at 4 and 6 weeks after pollination. Fertilization had taken place at 4 weeks after pollination with zygotes and free nuclear endosperm visible. There was a greater proportion of healthy, fertilized ovules in the cross- compared with the self-pollination treatment, and approx. half the ovules examined from both pollen treatments were not fertilized or were degenerating. By 6 weeks after pollination a few zygotes were starting to divide. The number of healthy, fertilized ovules was still greater in the cross-pollination treatment, but the number of healthy fertilized ovules was lower in both treatments compared with 4 weeks after pollination, and many ovules were degenerating. Fertilized ovules were significantly larger than non-fertilized or degenerating ovules and this difference was detectable by eye at 6 and 8 weeks after pollination. The mechanism of self-incompatibility appears to have both late pre- and post-zygotic components.

Item Type: Article
Authors/Creators:Pound, LM and Wallwork, MAB and Potts, BM and Sedgley, M
Keywords: Eucalyptus globulus ssp. globulus, Tasmanian blue gum, self-incompatibility, controlled pollination, bright field microscopy, embryology, fertilization, ovule, zygote
Journal or Publication Title: Annals of Botany
ISSN: 0305-7364
DOI / ID Number: 10.1093/aob/mcf089
Additional Information:

BM Potts. © The definitive version is available at http://www.sciencedirect.com

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