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Abstract

Olfactory ensheathing cells (OECs) have been shown previously to express Toll-like receptors and to respond to bacteria by translocating nuclear factor-kB from the cytoplasm to the nucleus. In this study,we show that OECs extended significantly more pseudopodia when they were exposed to Escherichia coli than in the absence of bacteria (p=0.019). Coimmunoprecipitation showed that E. coli binding to OECs was mediated by Toll-like receptor 4. Lyso-
Tracker, a fluorescent probe that accumulates selectively
in lysosomes, and staining for type 1 lysosomeassociated
membrane proteins demonstrated that endocytosed FITC-conjugated E. coli were translocated to lysosomes. They appeared to be subsequently broken down, as shown by transmission electron microscopy. No obvious adherence to the membrane and less phagocytosis was observed when
OECs were incubated with inert fluorescent microspheres.
The ability of OECs to endocytose bacteria supports the notion that OECs play an innate immune function by protecting olfactory tissues from bacterial infection.

Item Type:	Article
Authors/Creators:	Leung, YK and Chapman, JA and Harris, JA and Hale, DM and Chung, RS and West, AK and Chuah, MI
Keywords:	olfactory ensheathing cells - chemotaxis - endocytosis - innate immunity - ultrastructure
Journal or Publication Title:	Cellular and Molecular Life Sciences
ISSN:	1420-682X
DOI / ID Number:	10.1007/s00018-008-8184-1
Additional Information:	The original publication is available at www.springerlink.com
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