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Interference of some matrix ions in cation-exchange chromatography


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Divjak, B, Goessler, W, Haddad, PR and Novic, M 2003 , 'Interference of some matrix ions in cation-exchange chromatography' , Journal of Chromatography A, vol. 1008, no. 1 , pp. 89-96 , doi: 10.1016/S0021-9673(03)00976-2.

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Ion chromatographic analysis of ions in samples containing complex matrix composition strongly depends on the on-column co-processes caused by sample matrix components. In the present publication studies of different separation phenomena in cation-exchange chromatography are described. The studies were performed at ‘non-linear’ chromatographic conditions, when the concentration of matrix (interfering) ions significantly exceeded the concentration of the eluent ions. During the research work, the processes already identified in anion-exchange chromatography, i.e. self-elution, on-column change of the eluent and sample-induced micro-gradient elution were used to explain the chromatographic behavior of alkaline and earth-alkaline cations when samples with high matrix cation concentration were analyzed. When present at higher concentrations, the ammonium cation was found to cause self-elution (NH4+ fraction) as well as on-column eluent neutralization due to its ability to diffuse into/back from the porous core of the stationary phase (NH3 fraction). Co-elution of a matrix component and analytes of interest caused spectroscopic interferences that additionally influenced the peak shape of each individual analyte.

Item Type: Article
Authors/Creators:Divjak, B and Goessler, W and Haddad, PR and Novic, M
Keywords: Alkaline metals; Alkaline earth metals; Inorganic cations; Matrix effects; Gradient elution, sample-induced
Journal or Publication Title: Journal of Chromatography A
ISSN: 0021-9673
DOI / ID Number: 10.1016/S0021-9673(03)00976-2
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