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Ion-exclusion chromatography with conductimetric detection of aliphatic carboxylic acids on a weakly acidic cation-exchange resin by elution with benzoic acid–β-cyclodextrin

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Tanaka, K, Mori, M, Xu, Q, Helaleh, MIH, Ikedo, M, Taoda, H, Hu, W, Hasebe, K, Fritz, JS and Haddad, PR 2003 , 'Ion-exclusion chromatography with conductimetric detection of aliphatic carboxylic acids on a weakly acidic cation-exchange resin by elution with benzoic acid–β-cyclodextrin' , Journal of Chromatography A, vol. 997, no. 1-2 , pp. 127-132 , doi: 10.1016/S0021-9673(03)00545-4.

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Abstract

In this study, an aqueous solution consisting of benzoic acid with low background conductivity and β-cyclodextrin (β-CD) of hydrophilic nature and the inclusion effect to benzoic acid were used as eluent for the ion-exclusion chromatographic separation of aliphatic carboxylic acids with different pKa values and hydrophobicity on a polymethacrylate-based weakly acidic cation-exchange resin in the H+ form. With increasing concentration of β-cyclodextrin in the eluent, the retention times of the carboxylic acids decreased due to the increased hydrophilicity of the polymethacrylate-based cation-exchange resin surface from the adsorption of OH groups of β-cyclodextrin. Moreover, the eluent background conductivity decreased with increasing concentration of β-cyclodextrin in 1 mM benzoic acid, which could result in higher sensitivity for conductimetric detection. The ion-exclusion chromatographic separation of carboxylic acids with high resolution and sensitivity was accomplished successfully by elution with a 1 mM benzoic acid–10 mM cyclodextrin solution without chemical suppression.

Item Type: Article
Authors/Creators:Tanaka, K and Mori, M and Xu, Q and Helaleh, MIH and Ikedo, M and Taoda, H and Hu, W and Hasebe, K and Fritz, JS and Haddad, PR
Keywords: Carboxylic acids; Benzoic acid; Cyclodextrins; Mobile phase composition
Journal or Publication Title: Journal of Chromatography A
ISSN: 0021-9673
DOI / ID Number: 10.1016/S0021-9673(03)00545-4
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