PDF 314.pdf.pdf | Request a copy Full text restricted Available under University of Tasmania Standard License.

Abstract

New findings are reported on simple ways to modify an ordinary HPLC column to obtain efficient ion chromatographic (IC) separations. Permanently coating a column with an ionic surfactant is known to produce an effective column for IC. We now show that incorporation of a nonionic surfactant in the coating, or coating in separate layers, results in a dramatic reduction of ion retention times and gives sharper peaks. Dynamic coating by incorporating a small amount of an alcohol, diol or zwitterion in the aqueous mobile phase permits good separations of alkanecarboxylic acids. A mobile phase containing a quaternary ammonium cation and a zwitterion anion provides excellent separations of common anions on a silica C18 column. An aqueous eluent containing a mixture of a zwitterion 4-(2-hydroxyethyl) acid and methanesulfonic acid can be used in conjunction with a standard cation exchange column. After passing through a membrane suppressor, the mobile phase has a slightly acidic pH, permitting divalent transition metal ions (as well as others) to be detected by conductivity.

Item Type:	Article
Authors/Creators:	Fritz, JS and Zhu, Y and Haddad, PR
Keywords:	Surfactants; Inorganic anions; Inorganic cations; Organic acids; Coated columns; Dynamic coating
Journal or Publication Title:	Journal of Chromatography A
ISSN:	0021-9673
DOI / ID Number:	10.1016/S0021-9673(03)00114-6
Additional Information:	The definitive version is available at http://www.sciencedirect.com
Item Statistics:	View statistics for this item

Actions (login required)

Item Control Page