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Vacancy ion-exclusion chromatography of aromatic carboxylic acids on a weakly acidic cation-exchange resin


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Helaleh, MIH, Tanaka, K, Mori, Masanobu, Xu, Q, Taoda, H, Ding, MY, Hu, W, Hasebe, K and Haddad, PR 2003 , 'Vacancy ion-exclusion chromatography of aromatic carboxylic acids on a weakly acidic cation-exchange resin' , Journal of Chromatography A, vol. 997, no. 1-2 , pp. 139-144 , doi: 10.1016/S0021-9673(03)00115-8.

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Determination of aromatic carboxylic acids by conventional ion-exclusion chromatography is relatively difficult and methods generally rely on hydrophobic interaction between the solute and the resin. To overcome the difficulties in determining aromatic carboxylic acids a new approach is presented, termed vacancy ion-exclusion chromatography, which is based on use of the sample as mobile phase and an injection of aqueous 10% methanol onto a weakly acidic cation-exchange column (TSKgel OApak-A). Highly sensitive conductivity detection occurred with sharp and well-shaped peaks, leading to very efficient separations. The effects of sulfuric acid concentration added to the mobile phase, flow-rate, and column temperature on the retention volume of tested aromatic carboxylic acids was investigated. Retention times were found to be affected by the concentration of the analytes in the mobile phase and to some extent also by the addition of an organic modifier such as methanol to the injected water sample. Separation of sulfuric acid (SA), naphthalenetetracarboxylic acid (NTCA), phthalic acid (PA) and benzoic acid (BA) was satisfactory using this new approach. Detection limits were 0.66, 0.67, 0.42 and 0.86 μM and detector responses were linear in the range 1–100, 1–80, 2.5–100 and 10–40 μM, for SA, NTCA, PA and BA, respectively. Precision for retention times was 0.36% and for peak areas was 1.5%.

Item Type: Article
Authors/Creators:Helaleh, MIH and Tanaka, K and Mori, Masanobu and Xu, Q and Taoda, H and Ding, MY and Hu, W and Hasebe, K and Haddad, PR
Keywords: Carboxylic acids; Vacancy ion-exclusion chromatography
Journal or Publication Title: Journal of Chromatography A
ISSN: 0021-9673
DOI / ID Number: 10.1016/S0021-9673(03)00115-8
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