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Highly sensitive indirect photometric detection of cations by capillary electrophoresis with the cationic dye chrysoidine


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Johns, CA, Macka, M and Haddad, PR 2003 , 'Highly sensitive indirect photometric detection of cations by capillary electrophoresis with the cationic dye chrysoidine' , Journal of Chromatography A, vol. 997, no. 1-2 , pp. 87-94 , doi: 10.1016/S0021-9673(03)00062-1.

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The cationic dye, chrysoidine, has been used for the first time as a probe for the indirect photometric detection of cations. The dye has been used as a probe at concentrations of 5 mM, which is roughly an order of magnitude higher than for other cationic dyes used previously for the same purpose, in order to minimise electromigrational dispersion. Baseline instability was minimised by a combination of coating the capillary with poly(ethyleneimine), addition of a neutral polymer to the electrolyte, and the application of a small amount (20 mbar) of hydrodynamic pressure during the separation. Separation of a mixture containing alkali metals, alkaline earths, transition metals and lanthanides was achieved by the addition of 2-hydroxyisobutyric and lactic acid as complexing agents. Excellent peak shapes were observed over a wide range of analyte mobilities due to the moderate mobility of the probe. The high absorptivity (26 733 l mol−1 cm−1) provided by chrysoidine in comparison with typically used, less absorbing probes, was reflected in limits of detection which were typically less than 0.5 μM. These are amongst the lowest reported using hydrodynamic injection without the use of large volume stacking methods. The use of 2-hydroxyisobutyric and lactic acids as complexing agents at pH values close to their pKa values provided suitable buffering which was highlighted by very good reproducibility of migration time, corrected peak area and peak height.

Item Type: Article
Authors/Creators:Johns, CA and Macka, M and Haddad, PR
Keywords: Dyes, Chrysoidine; Inorganic cations; Organic acids; Indirect detection; Detection, electrophoresis
Journal or Publication Title: Journal of Chromatography A
ISSN: 0021-9673
DOI / ID Number: 10.1016/S0021-9673(03)00062-1
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