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Measurement of detector linearity and effective pathlength in capillary electrophoresis

journal contribution
posted on 2023-05-26, 10:32 authored by Johns, CA, Macka, M, Paul HaddadPaul Haddad
Detection linearity and effective pathlength are the two most important parameters for the performance of every photometric detector. Both parameters have been determined experimentally for a high-sensitivity detection cell (HSDC) and an extended light path capillary (ELPC) used in capillary electrophoresis. A series of solutions of increasing sodium chromate concentration was flushed through the capillary under pressure. A plot of sensitivity versus absorbance yielded both the linear range and effective pathlength of the detectors. The upper limit of the linear range was found to be 2190 mAU for the HSDC and 1490 mAU for the ELPC. The effective pathlengths were found to be 1068 and 128 ˜í¬¿m, respectively.

History

Publication title

LC-GC Europe

Volume

2003

Article number

May

Number

May

Pagination

290-293

ISSN

1471-6577

Publication status

  • Published

Rights statement

Copyright 2003 Advanstar Communications. All rights reserved

Repository Status

  • Restricted

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