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Measurement of detector linearity and effective pathlength in capillary electrophoresis

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Johns, CA, Macka, M and Haddad, PR 2003 , 'Measurement of detector linearity and effective pathlength in capillary electrophoresis' , LC-GC Europe, vol. 2003, No. May , pp. 290-293 .

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Abstract

Detection linearity and effective pathlength are the two most important parameters for
the performance of every photometric detector. Both parameters have been determined
experimentally for a high-sensitivity detection cell (HSDC) and an extended light path
capillary (ELPC) used in capillary electrophoresis. A series of solutions of increasing sodium
chromate concentration was flushed through the capillary under pressure. A plot of
sensitivity versus absorbance yielded both the linear range and effective pathlength of
the detectors. The upper limit of the linear range was found to be 2190 mAU for the HSDC
and 1490 mAU for the ELPC. The effective pathlengths were found to be 1068 and 128 μm,
respectively.

Item Type: Article
Authors/Creators:Johns, CA and Macka, M and Haddad, PR
Journal or Publication Title: LC-GC Europe
ISSN: 1471-6577
Additional Information:

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