Open Access Repository

Measurement of detector linearity and effective pathlength in capillary electrophoresis


Downloads per month over past year

Johns, CA, Macka, M and Haddad, PR 2003 , 'Measurement of detector linearity and effective pathlength in capillary electrophoresis' , LC-GC Europe, vol. 2003, no. May , pp. 290-293 .

[img] PDF
306.pdf.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.


Detection linearity and effective pathlength are the two most important parameters for
the performance of every photometric detector. Both parameters have been determined
experimentally for a high-sensitivity detection cell (HSDC) and an extended light path
capillary (ELPC) used in capillary electrophoresis. A series of solutions of increasing sodium
chromate concentration was flushed through the capillary under pressure. A plot of
sensitivity versus absorbance yielded both the linear range and effective pathlength of
the detectors. The upper limit of the linear range was found to be 2190 mAU for the HSDC
and 1490 mAU for the ELPC. The effective pathlengths were found to be 1068 and 128 μm,

Item Type: Article
Authors/Creators:Johns, CA and Macka, M and Haddad, PR
Journal or Publication Title: LC-GC Europe
ISSN: 1471-6577
Additional Information:

© 2003 Advanstar Communications. All rights reserved

Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page