Open Access Repository

Vacancy ion-exclusion chromatography of carboxylic acids on a strongly acidic cation-exchange resin

Tanaka, K, Ding, MY, Takahashi, H, Helaleh, MIH, Taoda, H, Hu, W, Hasebe, K, Haddad, PR, Mori, Masanobu, Fritz, JS and Sarzanini, C 2002 , 'Vacancy ion-exclusion chromatography of carboxylic acids on a strongly acidic cation-exchange resin' , Analytica Chimica Acta, vol. 474, no. 1-2 , pp. 31-35 , doi:

[img] PDF
296.pdf.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.


A new approach to ion-exclusion chromatography (IEC) is proposed to overcome the relatively poor conductivity detection response which occurs in IEC on when acids are added to the eluent in order to improve peak shape. This approach, termed vacancy IEC, requires the sample to be used as mobile phase and a sample of water to be injected onto a polystyrene/divinylbenzene (PS/DVB)-based strongly acidic cation-exchange column (TSKgel SCX). Vacancy peaks for each of the analytes appear at the retention times of these analytes. Highly sensitive conductivity detection is possible and sharp, well-shaped peaks are produced, leading to efficient separations. Retention times were found to be affected by the concentration of the analytes in the eluent, and also by the presence of an organic modifier, such as butanol in the mobile phase. Detection limits for oxalic, formic, acetic, propionic, butyric and valeric acids at S/N=3 were 0.04, 0.1, 0.47, 0.66, 0.71 and 0.82 μM, respectively, and linear ranges for some acids extended over two-orders of magnitude. Precision values for retention times were <0.57% and for peak height were <2.06%.

Item Type: Article
Authors/Creators:Tanaka, K and Ding, MY and Takahashi, H and Helaleh, MIH and Taoda, H and Hu, W and Hasebe, K and Haddad, PR and Mori, Masanobu and Fritz, JS and Sarzanini, C
Keywords: Ion-exclusion chromatography; Strongly acidic cation-exchange resin; Vacancy chromatography; Aliphatic carboxylic acids; Conductimetric detection
Journal or Publication Title: Analytica Chimica Acta
ISSN: 0003-2670
DOI / ID Number:
Additional Information:

The definitive version is available at

Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page