PDF 238.pdf.pdf | Request a copy Full text restricted Available under University of Tasmania Standard License.

Abstract

The absorption behaviour of zwitterionic surfactants from a micellar mobile phase onto a reversed-phase octadecylsilica (ODS) stationary phase was studied. Four betaine-type zwitterionic surfactants; 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS), 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-(N,N-dimethyldodecylammonio)-propane-sulfonate (C12N3S), and 3-(N,N-dimethylmyristylammonio)-propanesulfonate (C14N3S) were used as model surfactants. For all of these surfactants, similar results were observed. Breakthrough of micelles did not occur until the stationary phase had become coated at saturation with monomeric surfactant. Stationary phases prepared in this way were able to separate ions with water as mobile phase, with the separation being governed by the total amount of the surfactant passed over the stationary phase, rather than whether a micellar solution or a sub-micellar solution was used. Taken together, these data lead to the conclusion that the regardless of the type of surfactant solution in contact with the stationary phase only monomers became adsorbed until saturation of the stationary phase occurred. The amount of surfactant adsorbed onto the stationary phase at saturation was found to be 0.461, 0.480, 0.777, and 1.045 mmole column−1 for CHAPS, CHAPSO, C12N3S, and C14N3S, respectively.

Item Type:	Article
Authors/Creators:	Hu, W and Haddad, PR
Keywords:	Column liquid chromatography - Ion chromatography - Zwitterionic surfactants - Adsorption studies
Journal or Publication Title:	Chromatographia
ISSN:	0009-5893
DOI / ID Number:	10.1007/BF02789748
Additional Information:	The original publication is available at www.springerlink.com
Item Statistics:	View statistics for this item

Actions (login required)

Item Control Page