Open Access Repository

Pressurized-flow anion-exchange capillary electrochromatography using a polymeric ion-exchange stationary phase


Downloads per month over past year

Hilder, EF, Klampfl, CW and Haddad, PR 2000 , 'Pressurized-flow anion-exchange capillary electrochromatography using a polymeric ion-exchange stationary phase' , Journal of Chromatography A, vol. 890, no. 2 , pp. 337-345 , doi: 10.1016/S0021-9673(00)00612-9.

[img] PDF
236.pdf.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.


The feasibility of using capillary columns equipped with silica frits and packed with a polymer-based anion exchanger (Dionex AS9-HC) for CEC separations of inorganic anions has been investigated. Experiments using a conventional 25 cm packed bed, and mobile phase flow that is a combination of hydrodynamic and electroosmotic flow were used to demonstrate that by varying the applied voltage (electrophoresis component) or the concentration of the competing ion in the mobile phase (ion-exchange component), considerable changes in the separation selectivity could be obtained. Using an artificial neural network, this separation system was modelled and the results obtained used to determine the optimum conditions (9 mM perchlorate and −10 kV) for the separation of eight inorganic anions. When a short (8 cm) packed bed was used, with detection immediately following the packed section, the separation of eight test analytes in under 2.2 min was possible using pressure-driven flow and a simple step voltage gradient. A more rapid separation of these analytes was obtained by only applying high voltage (−30 kV), where many of the same analytes were separated in less than 20 s and with a different separation selectivity to that obtained in conventional ion-exchange or capillary electrophoresis separations.

Item Type: Article
Authors/Creators:Hilder, EF and Klampfl, CW and Haddad, PR
Keywords: Electrochromatography; Pressurized flow; Anion exchange; Stationary phase, CEC; Artificial neural network
Journal or Publication Title: Journal of Chromatography A
ISSN: 0021-9673
DOI / ID Number: 10.1016/S0021-9673(00)00612-9
Additional Information:

The definitive version is available at

Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page