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Capillary electrophoresis with end-capillary potentiometric detection using a copper electrode

journal contribution
posted on 2023-05-26, 16:03 authored by Macka, M, Gerhardt, G, Andersson, P, Bogan, DP, Cassidy, RM, Paul HaddadPaul Haddad
Potentiometric end-capillary detection in capillary electrophoresis has the advantage of relatively easy miniaturisation without having to compromise the concentration sensitivity. Potentiometric end-capillary detection using a copper electrode is also attractive because of the sensitive detection of many inorganic and organic UV-transparent ions and the ability to work in both direct and indirect mode. In this work, detection of a number of common anions in a tartrate electrolyte at pH 3 was studied. The influence of the end-capillary detection geometry on the detection performance was investigated. An end-capillary detection cell allowing the separation capillary to be changed without the need to realign the detection electrode was constructed and fitted into a commercial CE apparatus. Under the optimal configuration, which was a 25 m diameter copper electrode aligned coaxially with a 25 m capillary and positioned at a distance of about 25 m from the capillary end, excellent peak shapes were achieved and comparison with simultaneous on-capillary photometric detection showed no additional peak broadening. Good sensitivity was obtained, resulting in concentration limits of detection (LODs) in the low M range and mass LODs in the low amol range. Examples of separations of inorganic and organic anions are presented and the analytical potential of the detection method is assessed.

History

Publication title

Electrophoresis

Volume

20

Article number

12

Number

12

Pagination

2539-2546

ISSN

0173-0835

Publication status

  • Published

Rights statement

The definitive published version is available online at: http://interscience.wiley.com

Repository Status

  • Restricted

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