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Determination of protein synthesis in rainbow trout, Oncorhynchus mykiss, using a stable isotope


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Carter, CG, Owen, SF, He, ZY, Watt, PW, Scrimgeour, C, Houlihan, DF and Rennie, MJ 1994 , 'Determination of protein synthesis in rainbow trout, Oncorhynchus mykiss, using a stable isotope' , Journal of Experimental Biology, vol. 189 , pp. 279-284 .

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It has been suggested (Houlihan, 1991) that the consumption of 1 g of protein in a
variety of species of fish stimulates the synthesis of, approximately, an equal amount of
protein. Although synthesis of protein may account for as much as 40 % of the wholeanimal
oxygen consumption (Lyndon et al. 1992), only about 30 % of the synthesized
proteins are retained as growth (Houlihan et al. 1988; Carter et al. 1993a,b). Thus, one
focus of attention is the potential advantage gained by fish in allocating a considerable
proportion of assimilated energy to protein turnover in contrast to relatively low-cost,
low-turnover protein growth (Houlihan et al. 1993). Rates of protein synthesis in several
species of fish have been measured using radioactively labelled amino acids, frequently
given as a flooding dose (reviewed by Fauconneau, 1985; Houlihan, 1991). These
measurements cannot be made for longer than a few hours because of the decline in
specific radioactivity in the amino acid free pool. However, as protein synthesis rates vary
during the course of a day as a result of the post-prandial stimulation, and since
radiolabelled amino acid methodology is invasive, short-term and terminal, it has been
difficult to be certain of the relationship between protein growth measured in the long
term and protein synthesis rates measured in the short term. This paper addresses these
problems by developing a method using 15N in orally administered protein to measure
protein synthesis rates in fish over relatively long periods, the aim being to use procedures
that are as non-invasive and repeatable as possible.

Item Type: Article
Authors/Creators:Carter, CG and Owen, SF and He, ZY and Watt, PW and Scrimgeour, C and Houlihan, DF and Rennie, MJ
Journal or Publication Title: Journal of Experimental Biology
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