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Using RAPDs to Detect QTLs in an Interspecific F2 Hybrid of Eucalyptus


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Vaillancourt, RE, Potts, BM, Manson, AF, Eldridge, T and Reid, JB 1995 , 'Using RAPDs to Detect QTLs in an Interspecific F2 Hybrid of Eucalyptus', in BM Potts and NMG Borralho and JB Reid and RN Cromer (eds.), Eucalypt Plantations : Improving Fibre Yield and Quality - CRCTHF-IUFRO Conference, 19 - 24 February, 1995 , Hobart, Tasmania, Australia. , CRCTHF-IUFRO, Hobart, Tasmania, Australia., Hobart, Tasmania, Australia., pp. 430-433.

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The inheritance and linkage relationships of Random Amplitied Polymorphic DNA (RAPD) markers were examined
in seedlings of an interspecific hybrid between Eucalyptus gunnii and E. globulus ssp. globulus. One hundred and ten
RAPD markers were scored from 71 F2, 11 F1 and 10 backcrosses to E. gunnii. Nineteen percent of the RAPD markers
showed disturbed segregation in the F2, with most having a deficiency of E. globuluc bands (13 out of 18). The F2 had
high levels of morphological abnormalities and poor growth compared to parental controls.F1 and outcrossed F2 progenies.
Linkage analysis revealed fifteen linkage groups of three or more markers, 8 pairs and 13 unlinked markers
LOD < 3.0). Markers with disturbed segregation were clustered together. One normally segregating marker
(U63-695) was significantly associated with abnormal leaf morphology, branching and frost tolerance. A significant
proportion (ANOVA R2=18.5%) of the variation in frost tolerance in the F2 was explained by the marker. However,
abnormal leaf morphology and frost tolerance were not correlated. While interspecific hybridisation may be a powerful
means of generating large amounts of genetic variation, the detection and exploitation of QTLs may be complicated by
large and deleterious pleiotropic gene effects and biased by distorted segregation.

Item Type: Book Section
Authors/Creators:Vaillancourt, RE and Potts, BM and Manson, AF and Eldridge, T and Reid, JB
Keywords: QTL, Eucalyptus globulus, Eucalyptus gunnii, hybrid, F2
Publisher: CRCTHF-IUFRO, Hobart, Tasmania, Australia.
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