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Abstract

Platelet activation occurs in a variety of clinical situations in which it
directly contributes to the pathology. This study reports a simple flow
cytometric assay for platelet activation which measures platelet-derived
microparticles, activated platelets and platelet–monocyte complexes.
Pre- and post analytical conditions were investigated and optimized
and a normal range established on 20 healthy controls. Twenty patients
pre- and post percutaneous coronary intervention (PCI) were tested
with the technique. Soluble activation markers sCD40 ligand and sPselectin
and plasma phospholipid levels were measured in both groups.
There was a significant increase in activated platelets and platelet–
monocyte complexes between normal and pre-PCI (P = 0.005 and
0.0275, respectively) suggesting an activated state. There was a
significant fall in activated platelets post-PCI (P = 0.0027) which was
mirrored by a fall in soluble CD40 ligand, soluble P-selectin and plasma
phospholipid levels (P = 0.0066, <0.0001 and 0.0032, respectively)
consistent with antiplatelet therapy administered during the process.
This is a reliable and rapid method for the assessment of ex vivo platelet
activation which may be an aid in diagnosis and help guide therapy for
patients with thrombotic disease.

Item Type:	Article
Authors/Creators:	Pearson, L and Thom, J and Adams, MJ and Oostryck, R and Krueger, R and Yong, G and Baker, RI
Keywords:	Activated platelets, flow cytometry, percutaneous coronary intervention, platelet–monocyte complexes
Journal or Publication Title:	International Journal of Laboratory Hematology
ISSN:	1751-5521
DOI / ID Number:	https://doi.org/10.1111/j.1751-553X.2008.01059.x
Additional Information:	The original publication is available at http://www.interscience.wiley.com/
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